by Bajpai Labs · CRISPR & genomic optimization
Ranked CRISPR guides in days, not weeks.
GeneForge replaces manual guide design and slow CRO workflows with genome-wide screening that surfaces your best guide RNAs and codon-optimized constructs before a single synthesis order.
Delivery pipeline
- Genome-Wide Screening01500K+ guide variants · off-target search · tissue chromatin weighting
- Safety & Edit Refinement02Pathogenic loci exclusion · bystander prediction · manufacturability scoring
- Ranked Delivery03Top 10–20 sequences · validation playbook · manufacturing-ready files
- 4×
- Faster than manual CRO design
- 500K+
- Guide variants screened
- 10–14 days
- Typical delivery
Manual guide design is slow, risky, and misses off-targets.
Hand-picked guides miss cryptic off-target sites across the genome. One undetected cut at a pathogenic locus can derail an entire program.
CRO guide design and internal bioinformatics workflows take months. Manufacturing slots and IND timelines do not wait.
Manual design explores a tiny fraction of the search space. Most synthesized guides fail first-pass validation.
Genome-wide guide screening that ranks your best sequences before synthesis.
- 01
Upload your target
Provide a genomic locus, base editing objective, or therapeutic construct sequence.
- 02
Genome-wide pipeline runs
Guide enumeration, off-target screening, tissue chromatin weighting, and edit outcome simulation.
- 03
Ranked sequences delivered
Top 10–20 guides or constructs with safety tiers, validation playbook, and manufacturing files.
Built for gene editing decisions, not dashboards.
Every architectural choice in GeneForge serves one goal: get your team to the right guide sequences and constructs faster—with off-target safety built in.
Most CROs design a handful of guides by hand. GeneForge screens hundreds of thousands of variants genome-wide, so you synthesize only sequences worth validating.
Every guide is ranked by off-target risk tier before on-target score. Pathogenic loci and essential genes are excluded before ranking begins.
Research-driven methods from Bajpai Labs, delivered in days with full technical documentation your regulatory team can use.
You work with the architects who built the pipeline, not a sales team relaying requirements to a black box.
The science behind CRISPR optimization.
Published, peer-reviewed methods composed with proprietary scoring from Bajpai Labs.
DeepCRISPR ensemble scoring across 500K+ guide variants with tissue-specific chromatin accessibility weighting.
Cas-OFFinder, CHANGE-seq validated sites, and pathogenic loci exclusion with tiered risk classification.
BE-Hive and DeepABE ensemble for ABE/CBE guides with bystander edit prediction and edit outcome simulation.
AAV and lentiviral payload optimization with CAI scoring, mRNA structure prediction, and packaging constraint enforcement.
In silico indel and base edit distribution prediction for preclinical planning and regulatory documentation.
GC content, synthesis complexity, repeat motif detection, and immunogenic epitope filtering before delivery.
Methodology
Closed-Loop Edit Calibration
Each cycle narrows the candidate space using edit outcome data, not a one-pass screen.
Amplicon-seq and flow cytometry outcomes retrain scoring models, tightening predictions over time.
Closed-loop calibration catches guides that score well in silico but fail safety review in vitro.
Research rigor. Gene therapy speed.
GeneForge combines research-driven engineering from Bajpai Labs with genomic optimization expertise that most pure-software vendors lack.
- Genome-wide by default
Every engagement screens hundreds of thousands of variants—not a handful of hand-picked guides.
- Off-target safety built in
Pathogenic loci exclusion and tiered risk classification before any sequence reaches your lab.
- Manufacturing-ready output
Ranked sequences with validation playbooks and CMC-ready files your team can act on immediately.
- Direct line to leadership
You work with the architects who built the pipeline, not a sales team.
Gene editing services
Four pipelines. One editing platform.
From CRISPR guide design through codon optimization, each service shares the same genome-wide screening infrastructure and the same senior team.
All services- 00
CRISPR Guide Design
Genome-wide · Cas9/Cas12Start here500K+ guide variant screening, on-target activity prediction, genome-wide off-target analysis, and tissue-specific chromatin weighting. Delivers ranked guide RNAs with safety tiers.
Engagements typically $150K–$280K per programSee capabilities - 01
Off-Target Prediction & Safety Screening
Cas-OFFinder · CHANGE-seqGenome-wide off-target search with pathogenic loci exclusion, tiered risk classification, and amplicon sequencing panel design for confirmation.
Engagements typically $150K–$250K per programSee capabilities - 02
Base Editing Guide Optimization
ABE · CBE · bystander controlProtospacer enumeration, on-target edit prediction, bystander edit modeling, and edit outcome simulation for ex vivo and in vivo programs.
Engagements typically $200K–$320K per programSee capabilities - 03
Codon Optimization & Edit Simulation
AAV · lentiviral · expressionCodon optimization for gene therapy payloads, expression simulation, packaging constraint enforcement, and genome edit outcome modeling.
Engagements typically $250K–$400K per programSee capabilities
by Bajpai Labs
Ready to synthesize only your best guides?
30-minute intro call with the Bajpai Labs team. No pitch deck, just a scoping conversation.